Drug Testing
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MarijuanaScan saliva (fluid) drug testing kit
Easy to use, Rapid results, Less invasive, Best way to detect Marijuana use
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MarijuanaScan is an easy, visually-read screening test with a high degree of sensitivity and specificity, that does not require instrumentation.
Principle
MarijuanaScan tests are based on the principle of specific immunochemical reaction between antibodies and antigens to analyze particular compounds in human urine specimen. The assay relies on the competition for binding antibody between drug conjugate and free drug which may be present in the urine specimen being tested.
When a drug is present in the urine specimen, it competes with drug conjugate for the limited amount of antibody-dye conjugate. When the amount of drug is equal or more than the cut-off level of 50 ng/ml, it will prevent the binding of drug conjugate to the antibody.
Therefore, a positive urine specimen will not show a colored band on the test line zone, indicating a positive result, while the presence of a colored band indicates a negative result.
A control line is present in the test window to work as procedural control. This colored band should always appear on the control line zone if the test device is stored in good condition and the test is performed appropriately.
The control line serves to validate the test results.
Intended Use
MarijuanScan is a rapid, one step immunochromatographic assay for the qualitative determination of THC (Tetrahydrocannabinoid) and its metabolites in human urine. The test is used to screen the presence of 11-nor-Δ9-THC-9-COOH in human urine for a cutoff of 50 ng/ml.
The test provides only a presumptive result and should be confirmed by more specific alternate chemical methods such as gas chromatography/mass spectrometry (GC/MS) as recommended in AS/NZS 4308.
Positive results should be justified with compelling clinical evidence and professional judgement.
Summary and Explanation
The agents of Marijuana that cause various biological effects in humans are called cannabinoids. Cannabinoids are a central nervous stimulant that alters mood and sensory perceptions, produces loss of coordination, impairs short-term memory and produces symptoms of anxiety, paranoia, depression, confusion, hallucination and increased heart rate.
Large doses of cannabinoid could cause the development of tolerances and physiological dependency and lead to abuse. A tolerance to the cardiac and psychotropic effects can occur and withdrawal syndrome produces restlessness, insomnia, anorexia and nausea. Δ9-THC is the primary active ingredient in cannabinoids. The main metabolite excreted in the urine is 11-nor-Δ9-THC-9-COOH, which is found within hours of exposure and remains detectable in the urine for between 10-30 days after smoking.
All cannabinoids are controlled substances in Australia and the Australian Standard AS/NZS 4308 recommended cutoff level for Cannabinoid screening tests is 50 ng/ml in urine.
Expected Results
MarijuanaScan is a qualitative assay. It identifi es 11-nor-Δ9-THC-9-COOH in human urine at a concentration of 50 ng/ml or higher. The concentration of the 11-nor-Δ9-THC-9-COOH can not be determined by this assay.
The test is intended to distinguish negative results from presumptive positive results. All positive results must be confirmed using an alternate method, preferably GC/MS.
| 50 |
100 |
| 37.5 |
133 |
| 5000 |
1 |
| 15000 |
0.33 |
| 25000 |
0.20 |
Performance Characteristics
Sensitivity
The following table lists compounds that are detected by MarijuanaScan Tests:
Compounds
| 2000 mg/dl |
| 2000 mg/dl |
| 10 mg/dl |
| 4000 mg/dl |
| 10 mg/dl |
Interference testing
The following substances did not interfere with the MarijuanaScan test:
Specificity
The following compounds show no cross-reactivity at concentrations up to 100 µg/ml unless specified:
- Acetaminophen
- 4Acetamidophenol
- Acetylsalicylic acid
- Amikacin
- d,l-Amphetamine
- Amitriptyline
- Amobarbital
- Arterenol
- Aspartame
- Ascorbic acid
- Atrophine
- Benzoic acid
- Benzoylecgonine
- Butabarbital
- Caffeine
- Camphor
- Chloroquine
- Chlopheniramine
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- Cocaine
- Cortisone
- Deoxyephedrine
- Dextromethorphan
- Diazepam
- Digitoxin
- Digoxin
- Diphenhydramine
- Ecgonine
- Ecgonine methyl ester
- Ephedrine
- Epinephrine
- Gentisic acid
- Guaiacol glycer ester
- Histamine
- Hydrochlorothiazide
- Homatrophine
- Imipramine
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- Ibuprofen
- Isoproterenol
- Ketamine
- Lidocaine
- Methamphetamine
- 3,4±MDA
- 3,4±MDMA
- Meperidine
- Methadone
- Methaqualone
- Methylphenidate
- Morphine
- Neomycin
- Niacinamide
- Oxazepam
- Perphenazine
- Penicillin G
- Phencyclidine
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- Phenobarbital
- Phenylethylamine-a
- Phenylpropanolamine
- Promethazine
- Pseudoephedrine
- Quinine
- Rantidine
- Salicyclic acid
- Secobarbital
- Tetracycline
- Tetrahydrozoline
- Theophyline
- Thioridazine
- Trifl uoperazine
- Tryptophan
- Tyramine
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To learn more about alcohol and drug testing please contact us - ph 1800 640 075 free call.
References
- Urine Testing for Drugs of Abuse, NIDA Research Monograph 73, (1986).
- Blum, K., Handbook of Abusable drugs, Gardener Press, Inc., NewYork, NY, 1st Ed., (1984).
- Baselt RC., Disposition of Toxic Drugs and Chemicals in Man, 3rd Ed.,Cgicago, IL. Year Book Medical Publishers Inc., 780-783, (1990).
- Mandatory Guidelines for Federal Workplace drug Testing Programs, Fed. Reg. 53(69):11970-89 (1988).
- Australian Standard AS/NZS 4308.
How the MarijuanaScan Drug Testing device is used
Test Procedure
- Collect at least 30 ml of urine.
- Remove the test cassette from the foil pouch. DO NOT break the seal of the pouch until ready to begin testing.
- Place the test cassette on a flat surface.
- Dip the transfer pipette in the specimen and depress the bulb to withdraw a sample.
- Hold the pipette in a vertical position over the sample well (S) of the test cassette and deliver 2-3 drops of urine into the sample well.
- Read the results at 5-8 minutes
Interpretation of Results
• Negative:
The appearance of a Control line (C) in the test
window and a line in the Test Zone (T) indicates
a negative result. The color intensities of the
Control line and Test Zone line may not be
the same. A negative result does not indicate
the complete absence of THC in the specimen, it
only indicates that the levels of THC are below
the Australian Standards cut-off level of 50ng/ml.
• Positive:
The appearance of only a Control line (C) in
the test window and no line next to the Test Zone
(T) indicates that the result is positive. This is
an indication that the THC in the specimen is at
or above the Australian Standards cut-off level of
50ng/ml.
• Invalid:
A distinct Control line (C) should always appear.
If there is no line formation in the Control line
(C) position, the test result is invalid. Retest the
sample with a new device.
• Note: Any colour line in the test area, no matter
how faint, indicates a negative result. There is
no meaning attributed to line colour intensity or
width. The result must be interpreted at 5-8
minutes. Do not interpret the results after this
time. To avoid confusion, discard the test device
after reading the result.
Storage and Stability
The test device should be stored at 2° to 30°
C and
will be effective until the expiration date.
Precautions
- For in vitro diagnostic and forensic use only.
- Do not use the product beyond the expiration date.
- Handle all specimens as potentially infectious.
- Do not open foil pouch until it is ready to be tested.
- Use a new urine specimen cup for each sample to avoid cross contamination.
Quality Control
1. The control band is an internal reagent and procedural control. It will appear if the test has been performed correctly and the reagents are reactive.
2. Control standards can be used to validate reagent performance and establish test reliability. Controls which are not provided with this test are commercially available.
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